Neural Optical and Electrical Stimulation

Neural Optical and Electrical Stimulation Application
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While neural cultures are often spontaneously active, electrical and optical stimulation offers enhanced control of neural activity. Electrical stimulation can be used to study network connectivity, evaluate excitability, and reduce variability between replicates. Optogenetics can be used to further customize an experiment with the ability to stimulate or suppress activity within specific subpopulations of a culture.

The Maestro MEA platform provides noninvasive electrical activity recordings across each neural network, while the Lumos optical stimulation system delivers precisely controlled light. Together, these devices seamlessly pair to unlock entirely new assay capabilities, in a high-throughput format.

Powering neurons with light: optogenetic control of two neural populations
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Using the Lumos optical stimulator and Axion's multiwell Maestro MEA system to study neural network activity with optogenetics.  Neurons on the left side of the well have been transduced with a blue light-activated ion channel (channel rhodopsin, ChR2), the neurons on the right have not. Watch as blue light induces activity in the ChR2-labeled left-side neural population, that then spreads to, and activates the unlabeled right-side neural population.  After the addition of a cocktail of synaptic blockers the neural cells on the left can no longer drive the cells on the right to fire.

 

Click here to download the Axion BioSystems Neural Activity Brochure