What is Lux?
Lux
The Lux™ (Lux3™) is a miniaturized live-cell analysis platform designed to help researchers monitor cell cultures directly from the incubator using automated brightfield and fluorescence live-cell imaging. Visualize cell morphology and behavior in real-time with an easy-to-use system with fast and reliable data analysis.
Key Features
Single-well live-cell imaging from your incubator
>> Track complex biology in brightfield and fluorescence
From label-free to fluorescence-based cell tracking, the Axion Lux can help you to see and learn more.
>> Capture every moment, without crowding your incubator
The Lux operates within an incubator, automatically capturing timelapse images as your cells grow in an optimal environment. The compact device is perfect for labs with limited incubator space.
>> Monitor your cells and analyze remotely
The AxIS Vue™ software allows you to monitor your cells and perform data analysis from your desktop.
>> Get started quickly
A short training will get you up and running with the Axion Lux systems. The devices are easy to install and require no maintenance or calibration.
>> Expand to meet your needs
Link multiple Lux devices together to simultaneously monitor multiple culture vessels.
Overview
Lux overview
The Lux3 (Lux3 BR and Lux3 FL) allows you to visualize complex cellular biology with ease. Brightfield (BR and FL) and fluorescence (FL only) imaging combined with advanced software tools and intuitive design make the Lux3 an essential tool for every laboratory. Reveal the detailed kinetics of cell proliferation, cell migration, and morphology, and evaluate cell health for a deeper insight into complex cell behavior. For higher throughput, full-plate imaging, check out the Omni™ or Omni Pro 12™ live-cell imaging platforms.
Software/Algorithms
The Lux platform has three available software modules to match your assay needs:
Confluency Module – Know exactly when to passage your cells or track cell growth and death in your experiments with brightfield confluency.
Fluorescence Module – Add fluorescent assay capabilities with fluorescence confluency, intensity, and object detection.
Scratch Assay Module – Investigate the progression of wound closure over time.
FAQ:
The Lux is a compact digital microscope platform that utilizes brightfield and fluorescence microscopy to capture automated high-quality images of living cells from inside an incubator. Samples are illuminated using an LED and recorded with a fixed camera positioned below the sample stage. The images are uploaded to the Axion Portal where they can be analyzed using our image analysis algorithms or 3rd party software. The Lux platform includes two systems, the Lux3 BR with only brightfield capabilities, and the Lux3 FL with both brightfield and fluorescence.
What type of image analysis modules can I use?
The Lux is compatible with the following modules: Confluency Module, Scratch Assay Module (i.e. collective cell migration), and Fluorescent Module. Users always have the option to download the raw data and perform their own analysis on 3rd party software.
Can the Lux platform be used inside a cell culture incubator?
Yes, the Lux systems are designed to be used inside a cell culture incubator. The hardware and electronics can operate at 5 – 40°C and between 20 – 95% humidity.
What culture vessels are compatible with the Lux systems?
Any transparent culture vessel that is lower than 55 mm (2.2’’) (height of the light arc) can be scanned. Some examples include 6- to 384-well plates, Petri dishes, T25 to T225, microfluidic chips, and Petri dishes.
