Omni
The Omni™ is a live-cell analysis platform capable of continuous multiwell imaging directly from the incubator. Gain unprecedented access to dynamic biology and track cell health and function with automated, high-quality brightfield and fluorescent images of living cells. The versatile device can scan all types of flasks, microplates, or custom microfluidic chips while providing fast, reliable data analysis with powerful and intuitive software.
Key Features
Dynamic cell behavior imaged in your incubator
>> Track every moment, straight from your incubator
The Omni operates within an incubator, automatically capturing images as your cells grow in their optimal environment.
>> Assay your cells in brightfield and fluorescence
From label-free cell monitoring to fluorescence-based assays, the Omni adds dynamic visual results to any experiment.
>> See every cell
Capture high-resolution images of the entire culture without disturbing the cells with whole-well brightfield imaging.
Kinetic, real-time analysis made simple
See beyond the static with live-cell imaging. While endpoint assays only capture data at a single timepoint, live-cell imaging enables continuous observation, revealing the progression of cellular events as they unfold.
Multiplex your assay: Maestro + Omni
>> Platform cross-compatibility lets you view live-cell imaging alongside real-time, functional data to enhance your analysis
>> Monitor electrode coverage and cell morphology to ensure consistent, quality results
>> Experience no-hassle tracking—the Axion Portal automatically links data via barcode
Overview
The Omni overview
The Omni platform is designed to simplify and accelerate the acquisition of complex biological data. Using brightfield and fluorescence imaging combined with advanced software tools, the Omni can help you to examine cell health and function noninvasively and in real time.
Software/Algorithms
The Omni platform has six different software modules available to meet your assay needs:
Confluency Module – Know exactly when to passage your cells or track cell growth and death in your experiments with brightfield confluency.
Clonogenic Assay Module – Monitor and quantify cell proliferation and colony formation to study the effects of cytotoxic and immunotherapeutic agents.
Fluorescence Module – Add fluorescent assay capabilities with fluorescence confluency, intensity, and object detection.
iPSC Module – For monitoring the growth of pluripotent stem cells in a feeder-free culture environment.
Organoid Analysis Module – Identify, track, and analyze organoids across an array of culture vessels using an advanced machine-learning algorithm.
Scratch Assay Module – Investigate the progression of wound closure over time.
Cross-compatible Platforms
View data collected with your Omni within the software of these compatible platforms:
Maestro MEA™ – See whole-plate images of your cultures next to real-time activity measurements. Confirm electrode coverage and culture health with just a few clicks. Compatible with the Maestro Pro™ and Edge™ systems.
Maestro Z™ – Complement your impedance assays with whole-plate images of your cultures. Monitor electrode coverage and culture health over time with just a few clicks. Compatible with the Maestro ZHT™, TrayZ™, and Z™ systems.
Omni live-cell imager FAQ
How does the Omni work?
Samples are illuminated using an LED and recorded with a moving camera positioned below the sample stage. During brightfield acquisition, the camera scans the sample stage and acquires a series of sequential images. One complete brightfield scan generates approximately 7850 snapshots. These are stitched to form an image with a surface area of 86 mm × 124 mm (3.4’’ × 4.9’’). When acquiring fluorescence images, users can choose how many snapshots of a defined location within the well to record. The images are uploaded to the Cloud where they can be analyzed using our image analysis algorithms or third-party software.
What type of image analysis modules can I use?
The Omni is compatible with the following modules: Brightfield/fluorescence Cell Confluence Analysis Algorithm, Scratch Assay (i.e., collective cell migration) Analysis Algorithm, Clonogenic Assay Algorithm, and Fluorescent Object Count. Users always have the option to download the raw data and perform their own analysis on third-party software.
Can the Omni platform be used inside a cell culture incubator?
Yes, the Omni systems are designed to be used inside a cell culture incubator. The hardware and electronics can operate at 5-40°C and between 20-95% humidity.
What culture vessels are compatible with the Omni systems?
Any transparent culture vessel that is lower than 55 mm (2.2’’) (height of the light arc) can be scanned. Some examples include 6- to 384-well plates, Petri dishes, and T25 to T225 flasks. Users should keep in mind that the size of the scan area is limited to 86 mm × 124 mm (3.4’’ × 4.9’’).
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