Authors: Ujwal Boddeti, Jenna Langbein, Darrian McAfee, Marcelle Altshuler, Muzna Bachani, Hitten P. Zaveri, Dennis Spencer, Kareem A. Zaghloul, and Alexander Ksendzovsky
Frontiers in Network Physiology, 02 September 2024
Researchers use Axion’s hands-free Maestro Pro to evaluate seizure-like activity in neuron-glia co-cultures in vitro.
More than 30% of patients with drug-resistant epilepsy continue to experience seizures even after resective surgery, likely due to seizure network development. Seizure network development, however, has been difficult to assess in vivo, as most studies are restricted to a snapshot in time. In this study, researchers developed a novel in vitro MEA seizure model to examine these networks over time.
Neuron-glia co-cultures were continuously monitored using Axion BioSystems’ noninvasive Maestro Pro MEA, with chronic 4-AP treatment used to generate seizure-like activity. Chronic 4-AP treatment induced increased functional activity and network alterations that can be clearly visualized and quantified to serve as in vitro seizure network model. Overall, the authors conclude that this model can be used to study seizure network development, neuromodulation, and potential anti-epileptic therapeutic strategies.
