Clonogenic (or colony formation) assay is an in vitro cell survival assay that evaluates the ability of a single cell to form a colony. The assay has been widely used to test the efficacy of cancer drugs, gene therapies, and other cytotoxic agents; however, the manual examination and quantification of formed colonies can be a time-consuming and laborious task. An automated approach allows you increase clonogenic assay throughput and reproducibility and gain a kinetic insight into the effect of drugs on cell growth.
Evaluation of chemotherapies using clonogenic assays
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Automatically measure the number of colonies in a well>
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Determine a clonogenic assay tipping point by size and number>
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Measure dose-dependent effects on colony growth >
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Application Note: Quantifying chemotoxicity on cancer cell colonies>
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AI-driven quantification of colony formation>
Purpose: To demonstrate whole-well quantification of colony number following anti-cancer (Dox) treatment. Plated at low densities, a whole-well quantification is necessary to ensure accurate assessment of the surviving fraction.
The Clonogenic Assay module overlays individual colonies (A) and calculates the number of colonies (B) in a well.
CHO-K1 cells were treated with a range of Dox concentrations. The formation of colonies over time was analyzed on the Omni platform using the Colony Assay module.
Result: The individual colonies were marked (A) and counted (B) in each well automatically. At these low densities whole-well imaging is crucial for obtaining accurate counts.
